{"id":248,"date":"2025-06-11T13:46:17","date_gmt":"2025-06-11T13:46:17","guid":{"rendered":"https:\/\/analizesanocare.ro\/?post_type=product&p=248"},"modified":"2025-08-08T13:57:02","modified_gmt":"2025-08-08T13:57:02","slug":"acid-14-metil-imidazolacetic-metabolit-histamina","status":"publish","type":"product","link":"https:\/\/analizesanocare.ro\/en\/analiz\u0103\/acid-14-metil-imidazolacetic-metabolit-histamina\/","title":{"rendered":"Acid 1,4\u2013metil-imidazolacetic (metabolit histamina)"},"content":{"rendered":"
\n

Informa\u0163ii generale<\/strong> \u2013 Acid 1,4\u2013metil-imidazolacetic (metabolit histamina)<\/strong><\/h2>\n

Histamina, o amin\u0103 biogen\u0103 vasoactiv\u0103, constituie probabil cel mai cunoscut mediator stocat \u00een granulele mastocitelor \u015fi bazofilelor cu rol central \u00een r\u0103spunsul alergic. La om mastocitele con\u0163in aproximativ 2-5 pg histamin\u0103\/celul\u0103, \u00een timp ce bazofilele prezint\u0103 o cantitate mai redus\u0103 (1 pg\/celul\u0103).<\/p>\n

Mediatorii mastocitari, inclusiv histamina, predomin\u0103 \u00een r\u0103spunsul alergic precoce; bazofilele reprezint\u0103 sursa major\u0103 de histamin\u0103\u00a0\u00een r\u0103spunsul alergic de faz\u0103 tardiv\u01032<\/sup>.<\/p>\n

Histamina este sintetizat\u0103 \u00een aparatul Golgi prin decarboxilarea aminoacidului histidin\u0103 \u00een prezen\u0163a enzimei histidin-decarboxilaza. Histamina are o sarcin\u0103 electric\u0103 pozitiv\u0103 \u015fi se asociaz\u0103 cu\u00a0 grup\u0103rile carboxil ale proteoglicanilor din granulele mastocitelor. Dup\u0103 exocitoz\u0103, histamina disociaz\u0103 de proteoglicani la un pH neutru.<\/p>\n

O dat\u0103 eliberat\u0103 \u00een spa\u0163iul extracelular, histamina este metabolizat\u0103 rapid (timp de \u00eenjum\u0103t\u0103\u0163ire ~ 15-20 minute) prin metilare \u015fi oxidare. Metilarea, catalizat\u0103 de enzima histamin-metil-transferaz\u0103, constituie principala cale de metabolizare la nivelul c\u0103ilor respiratorii. Produsul ini\u0163ial, N-metilhistamina, poate fi excretat prin rinichi sau poate fi deaminat sub ac\u0163iunea monoaminoxidazei rezult\u00e2nd acidul 1,4 metil-imidazolacetic (MIIA). Histamina mai poate fi oxidat\u0103 si de c\u0103tre diamin-oxidaza (histaminaza) cu generarea acidului imidazolacetic (IAA).<\/p>\n

Datorit\u0103 metabolismului rapid este dificil s\u0103 se determine histamina \u00een plasm\u0103; \u00een schimb m\u0103surarea histaminei \u015fi a metaboli\u0163ilor s\u0103i \u00een urina din 24 ore constituie o alternativ\u0103 eficient\u0103 pentru testare ca urmare a faptului c\u0103 valorile acestora se men\u0163in mult mai mult timp crescute1<\/sup>.<\/p>\n

MIIA este principalul metabolit al histaminei (50% din histamin\u0103 este degradat\u0103 \u00een acest compus); din acest motiv reprezint\u0103 un marker cantitativ pentru eliberarea endogen\u0103 de histamin\u01037<\/sup>. Astfel, MIIA poate fi util \u00een confirmarea diagnosticului de anafilaxie6<\/sup>. De asemenea s-a constatat c\u0103 nivelurile urinare de acid metil-imidazol acetic se coreleaz\u0103 str\u00e2ns cu severitatea mastocitozei sistemice \u015f pot constitui un intrument util at\u00e2t pentru confirmarea diagnosticului c\u00e2t \u015fi pentru aprecierea evolu\u0163iei \u015fi a prognosticului3<\/sup>.<\/p>\n

Unele forme rare de tumori carcinoide gastrice se manifest\u0103 clinic printr-un tablou atipic mediat prin histamina eliberat\u0103 de c\u0103tre celulele tumorale; la ace\u015fti pacien\u0163i\u00a0determinarea de acid metilimidazol acetic poate fi util\u0103, \u00eempreun\u0103 cu cea de gastrin\u0103 \u015fi acid 5-hidroxi indolacetic5<\/sup>.<\/p>\n

Recomand\u0103ri pentru determinarea <\/strong>acidului 1,4 \u2013 metilimidazolacetic<\/strong><\/h2>\n

Evaluarea pacien\u0163ilor cu suspiciune de anafilaxie sau de mastocitoz\u0103 sistemic\u01033;6<\/sup>.<\/p>\n

Preg\u0103tire pacient <\/strong><\/h2>\n

Se va evita, cu 2 zile \u00eenaintea recolt\u0103rii, consumul de alimente cu un con\u0163inut ridicat \u00een histamin\u01031<\/sup>.<\/p>\n

Recipient de recoltare <\/strong>\u2013 vas de 2-3 litri \u015fi pahar de plastic de unic\u0103 folosin\u0163\u0103 pentru urin\u0103, pe care se noteaz\u0103 cantitatea total\u0103 de urin\u0103 din 24 ore4<\/sup>.<\/p>\n

Cantitate recoltat\u0103 <\/strong>\u2013 10 mL4<\/sup>.<\/p>\n

Cauze de respingere a probei <\/strong>\u2013 specimen care nu a fost p\u0103strat la 2-8\u00b0C; utilizarea de substan\u0163e conservante (produc interferen\u0163e)4<\/sup>.<\/p>\n

Prelucrare necesar\u0103 dup\u0103 recoltare <\/strong>\u2013 \u00een e\u015fantionul recoltat se vor ad\u0103uga c\u00e2teva pic\u0103turi de acid acetic glacial4<\/sup>.<\/p>\n

Stabilitate prob\u0103 <\/strong>\u2013 1 lun\u0103 la -20\u00b0C4<\/sup>.<\/p>\n

Metod\u0103<\/strong> \u2013 cromatografie de lichide cuplat\u0103 cu spectrometrie de mas\u0103 (LC\/MS)1<\/sup>.<\/p>\n

Valori de referin\u0163\u0103 Acid 1,4\u2013metil-imidazolacetic (metabolit histamina)<\/strong><\/h2>\n

<3.8 mg\/24 h4<\/sup>.<\/p>\n

Limite \u015fi interferen\u0163e<\/strong><\/div>\n
Ob\u0163inerea unei valori normale MIIA nu exclude un eveniment anafilactic6<\/sup>.<\/div>\n

 <\/p>\n

Bibliografie<\/strong><\/h2>\n
1. Florence Ida Hsu, Joshua A Boyce. Biology of Mast Cells and their Mediators. In Middleton\u2019s Allergy, Seventh Edition., Mosby Elsevier, 2009, 320.<\/div>\n
2. J. Andrew Grant, Saffana N. Hassan, Patricia A. Leonard. Mast Cell and Basophil-derived Mediators. In Atlas Of Allergic Diseases, 2nd Edition, Current Medicine LLC, 19.<\/div>\n
3. Karin Hartmann, Stefanie B Bruns and Beate M Henz. Mastocytosis: Review of Clinical and Experimental Aspects. In Journal of Investigative Dermatology Symposium Proceedings (2001) 6, 143\u2013147.<\/div>\n
4. Laborator Synevo. Referin\u0163ele specifice tehnologiei de lucru utilizate 2010. Ref Type: Catalog.<\/div>\n
5. Luigi Santacroce. Malignant Carcinoid Syndrome: Differential Diagnoses & Workup. www.emedicine.escape.com<\/a>. Reference Type: Internet Communication.<\/div>\n
6. Philip L Lieberman. Anaphylaxis. In Middleton\u2019s Allergy, Seventh Edition., Mosby Elsevier, 2009, 1041.<\/div>\n
7. Robert Hamilton, Anne Kagey-Sobotka. In Vitro Diagnostic Tests of IgE-Mediated Diseases. In Diagnostic Testing of Allergic Diseases, Marcel Dekker Inc, 2000, 99-100.<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"

Informa\u0163ii generale \u2013 Acid 1,4\u2013metil-imidazolacetic (metabolit histamina) Histamina, o amin\u0103 biogen\u0103 vasoactiv\u0103, constituie probabil cel mai cunoscut mediator stocat \u00een granulele mastocitelor \u015fi bazofilelor cu rol central \u00een r\u0103spunsul alergic. La om mastocitele con\u0163in aproximativ 2-5 pg histamin\u0103\/celul\u0103, \u00een timp ce bazofilele prezint\u0103 o cantitate mai redus\u0103 (1 pg\/celul\u0103). Mediatorii mastocitari, inclusiv histamina, predomin\u0103 \u00een […]<\/p>\n","protected":false},"featured_media":0,"template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[94,44],"product_tag":[],"class_list":["post-248","product","type-product","status-publish","product_cat-teste-pentru-monitorizarea-inflamatiei-alergice","product_cat-teste-specializate-de-alergologie-si-imunologie","first","instock","shipping-taxable","purchasable","product-type-simple"],"acf":[],"_links":{"self":[{"href":"https:\/\/analizesanocare.ro\/en\/wp-json\/wp\/v2\/product\/248","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/analizesanocare.ro\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/analizesanocare.ro\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:attachment":[{"href":"https:\/\/analizesanocare.ro\/en\/wp-json\/wp\/v2\/media?parent=248"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/analizesanocare.ro\/en\/wp-json\/wp\/v2\/product_brand?post=248"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/analizesanocare.ro\/en\/wp-json\/wp\/v2\/product_cat?post=248"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/analizesanocare.ro\/en\/wp-json\/wp\/v2\/product_tag?post=248"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}